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Fluorescent properties and

Hong GAO,Jie SONG,Shibin SHANG,Zhanqian SONG

《农业科学与工程前沿（英文）》 2017年 第4卷 第1期   页码 106-115 doi: 10.15302/J-FASE-2017133

摘要： A series of dehydroabietic acid-based diarylamines have been synthesized in order to investigate their fluorescent properties, photostability, cell toxicity and fluorescence imaging. The geometries as well as their molecular properties were optimized at the B3LYP/6-31G* level using Gaussian 03. The results indicate that molecular geometry, HOMO and LUMO energies, and energy gaps are important to predict absorption and fluorescent properties. Five of the compounds can be effectively taken up by human cervical carcinoma, human hepatocellular carcinoma SMMC-7721, human gastric cancer SGC-7901 and human lung adenocarcinoma A549 cells and strong blue fluorescent signals are detected in these cells. These compounds are potential candidates for fluorescent probes in biological diagnosis.

关键词： dehydroabietic acid-based diarylamine     DFT study     fluorescent probe     in vitro imaging    

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Recent advances in small molecule fluorescent probes for simultaneous imaging of two bioactive moleculesin live cells and <i>in vivoi>

Yongqing Zhou, Xin Wang, Wei Zhang, Bo Tang, Ping Li

《化学科学与工程前沿（英文）》 2022年 第16卷 第1期   页码 4-33 doi: 10.1007/s11705-021-2041-2

摘要： The interrelationships and synergistic regulations of bioactive molecules play pivotal roles in physiological and pathological processes involved in the initiation and development of some diseases, such as cancer and neurodegenerative and cardiovascular diseases. Therefore, the simultaneous, accurate and timely detection of two bioactive molecules is crucial to explore their roles and pathological mechanisms in related diseases. Fluorescence imaging associated with small molecular probes has been widely used in the imaging of bioactive molecules in living cells and due to its excellent performances, including high sensitivity and selectivity, noninvasive properties, real-time and high spatial temporal resolution. Single organic molecule fluorescent probes have been successively developed to simultaneously monitor two biomolecules to uncover their synergistic relationships in living systems. Hence, in this review, we focus on summarizing the design strategies, classifications, and bioimaging applications of dual-response fluorescent probes over the past decade. Furthermore, future research directions in this field are proposed.

关键词： bioactive molecules     fluorescent probes     in living cells and in vivo     review    

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Implications of static

John NSOR-ATINDANA, Maoshen CHEN, Liu WEI, Khin Myat NOE, Yue LI, Fang ZHONG

《农业科学与工程前沿（英文）》 2018年 第5卷 第3期   页码 340-350 doi: 10.15302/J-FASE-2018209

摘要：

Interest in understanding the digestion behavior of starch in the presence of dietary fibers is growing due to the ability of dietary component to control the release and absorption of glucose. This presents an outstanding opportunity to improve the quality of food products by incorporating dietary fiber into starchy food products. The physicochemical properties of different fibers and their behavior in the gastrointestinal tract (GIT) differ. To test the efficacy of these different fibers on starch digestion, static in vitro digestion models under conditions that mimic the human GIT are frequently used. Indeed, many efforts have been committed to the development of various static in vitro protocols for starch digestion. Though not considered as the gold standard in digestibility studies in food science and technology, static simulated models provide a useful alternative to in vivo techniques for rapid screening of the digestibility of food products under conditions that simulate the human GIT. This review presents the current status and development of digestion techniques for simulating digestion conditions in the human GIT, with particular interest on starch digestion in the presence of dietary fiber in the three phases of digestions including the oral, gastric and the intestinal steps. This summary can benefit investigators in developing static in vitro digestion models designed to simulate starch digestion with relevant values of the quantifiable parameters, including pH, enzymes and simulated digestive fluids.

关键词： dietary fiber     in vitro digestion     nutrition     simulation     starch    

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<i>Ni>-Positive ion activated rapid addition and mitochondrial targeting ratiometric fluorescent probesfor <i>in vivoi> cell H2S imaging

Yan Shi, Fangjun Huo, Yongkang Yue, Caixia Yin

《化学科学与工程前沿（英文）》 2022年 第16卷 第1期   页码 64-71 doi: 10.1007/s11705-021-2048-8

摘要： Heterocyclic compound quinoline and its derivatives exist in natural compounds and have a broad spectrum of biological activity. They play an important role in the design of new structural entities for medical applications. Similarly, indoles and their derivatives are found widely in nature. Amino acids, alkaloids and auxin are all derivatives of indoles, as are dyes, and their condensation with aldehydes makes it easy to construct reaction sites for nucleophilic addition agents. In this work, we combine these two groups organically to construct a rapid response site (within 30 s) for H S, and at the same time, a ratiometric fluorescence response is presented throughout the process of H S detection. As such, the lower detection limit can reach 55.7 nmol/L for H S. In addition, cell imaging shows that this probe can be used for the mitochondrial targeted detection of endogenous and exogenous H S. Finally, this probe application was verified by imaging H S in nude mice.

关键词： heterocyclic compound     hydrogen sulfide     ratiometric     mitochondrial targeted    

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SWIR Fluorescence Imaging <i>In Vivoi> Monitoring and Evaluating Implanted M2 Macrophages in Skeletal Article

Mo Chen, Yuzhou Chen, Sijia Feng, Shixian Dong, Luyi Sun, Huizhu Li, Fuchun Chen, Nguyen Thi Kim Thanh, Yunxia Li, Shiyi Chen, You Wang, Jun Chen

《工程（英文）》 doi: 10.1016/j.eng.2023.05.010

摘要：

Skeletal muscle has a robust regeneration ability that is impaired by severe injury, disease, and aging, resulting in a decline in skeletal muscle function. Therefore, improving skeletal muscle regeneration is a key challenge in treating skeletal muscle-related disorders. Owing to their significant role in tissue regeneration, implantation of M2 macrophages (M2Mø) has great potential for improving skeletal muscle regeneration. Here, we present a short-wave infrared (SWIR) fluorescence imaging technique to obtain more in vivo information for an in-depth evaluation of the skeletal muscle regeneration effect after M2Mø transplantation. SWIR fluorescence imaging was employed to track implanted M2Mø in the injured skeletal muscle of mouse models. It is found that the implanted M2Mø accumulated at the injury site for two weeks. Then, SWIR fluorescence imaging of blood vessels showed that M2Mø implantation could improve the relative perfusion ratio on day 5 (1.09 ± 0.09 vs 0.85 ± 0.05; p = 0.01) and day 9 (1.38 ± 0.16 vs 0.95 ± 0.03; p = 0.01) post-injury, as well as augment the degree of skeletal muscle regeneration on day 13 post-injury. Finally, multiple linear regression analyses determined that post-injury time and relative perfusion ratio could be used as predictive indicators to evaluate skeletal muscle regeneration. These results provide more in vivo details about M2Mø in skeletal muscle regeneration and confirm that M2Mø could promote angiogenesis and improve the degree of skeletal muscle repair, which will guide the research and development of M2Mø implantation to improve skeletal muscle regeneration.

关键词： In vivo     Short-wave infrared     Skeletal muscle     Macrophage     Regeneration    

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Effects of red and blue LEDs on

Lili CHEN, Xuzhang XUE, Yadong YANG, Fei CHEN, Jie ZHAO, Xiquan WANG, Alam Tariful KHAN, Yuegao HU

《农业科学与工程前沿（英文）》 2018年 第5卷 第2期   页码 197-205 doi: 10.15302/J-FASE-2018224

摘要： The objectives of this study were to investigate the effects of red and blue LEDs on growth and microtuberization of potato ( ) single-node cuttings. Explants were incubated under 6 light treatments: 100% red LEDs (R), 75% red LEDs+ 25% blue LEDs (3RB), 50% red LEDs+ 50% blue LEDs (RB), 25% red LEDs+ 75% blue LEDs (R3B), 100% blue LEDs (B) and white LEDs (W). Most of the growth and physiological parameters were significantly higher in 3RB than W. Enhancement of leaf area and chlorophyll concentrations were obtained in B. Leaf stomata were elliptical with the lowest density in 3RB. However, those in W were round in shape, and those with the smallest size and the highest density were observed in R. Most of the characteristics of microtuberization were also improved in 3RB. The combined spectra of red and blue LEDs increased the number of large microtubers. The fresh weight of individual microtubers in R and W were increased, but not their number. These results suggest that, of the treatments assessed, 3RB is optimal for the growth of potato plantlets and the combination of red and blue LEDs is beneficial for microtuberization.

关键词： blue LED     microtuber     plant tissue culture     potato plantlets in vitro     red LED    

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What will the future hold for artificial organs in the service of assisted reproduction: prospects and considerations

Mara Simopoulou, Konstantinos Sfakianoudis, Petroula Tsioulou, Anna Rapani, Polina Giannelou, Nikolaos Kiriakopoulos, Agni Pantou, Nikolaos Vlahos, George Anifandis, Stamatis Bolaris, Konstantinos Pantos, Michael Koutsilieris

《医学前沿（英文）》 2019年 第13卷 第6期   页码 627-638 doi: 10.1007/s11684-019-0697-5

摘要： Assisted reproduction provides a wide spectrum of treatments and strategies addressing infertility. However, distinct groups of infertile patients with unexplained infertility, congenital disorders, and other complex cases pose a challenge in fertilization (IVF) practices. This special cohort of patients is associated with futile attempts, IVF overuse, and dead ends in management. Cutting edge research on animal models introduced this concept, along with the development of artificial organs with the aim to mimic the respective physiological functions in reproduction. Extrapolation on clinical application leads to the future use of infertility management in humans. To date, the successful clinical application of artificial reproductive organs in humans is not feasible because further animal model studies are required prior to clinical trials. The application of these artificial organs could provide a solution to infertility cases with no other options. This manuscript presents an overview on the current status, future prospects, and considerations on the potential clinical application of artificial ovary, uterus, and gametes in humans. This paper presents how the IVF practice landscape may be shaped and challenged in the future, along with the subsequent concerns in assisted reproductive treatments.

关键词： artificial ovary     artificial uterus     artificial gametes     assisted reproduction     considerations     in vitro fertilization    

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Development of a new method for RMR and Q classification method to optimize support system in tunneling

Asghar RAHMATI,Lohrasb FARAMARZI,Manouchehr SANEI

《结构与土木工程前沿（英文）》 2014年 第8卷 第4期   页码 448-455 doi: 10.1007/s11709-014-0262-x

摘要： Rock mass classification system is very suitable for various engineering design and stability analysis. classification method is confirmed by Japan Highway Public Corporation that this method can figure out either strength or deformability of rock mass, further appropriating the amount of rock bolts, thickness of shotcrete, and size of pitch of steel ribs just after the blasting procedure. Based on these advantages of method, in this study, according to data of five deep and long tunnels in Iran, two equations for estimating the value of method from and classification systems were developed. These equations as a new method were able to optimize the support system for and classification systems. From classification and its application in these case studies, it is pointed out that the method for the design of support systems in underground working is more reliable than the and classification systems.

关键词： JH classification     Q and RMR classification     new method    

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抗白粉病基因 <i>Pm40i> 在我国“后 <i>Pm21i> 时代”小麦育种中的重要作用 Perspective

唐圣雯,胡玉亭,钟胜福,罗培高

《工程（英文）》 2018年 第4卷 第4期   页码 500-506 doi: 10.1016/j.eng.2018.06.004

摘要：

由小麦白粉菌[Blumeria graminis f. sp. tritici（Bgt）] 引起的小麦白粉病是一种重要的小麦叶部病害，对小麦的产量产生很大的影响。20 世纪80 年代，通过簇毛麦（Heuchera villosa）的6VS 染色体与小麦6AL 染色体易位将抗白粉病基因Pm21 转移到普通小麦中。最近，在一些地方发现了对Pm21 有毒的Bgt，虽然这些菌株的病理学特性还有待研究，但这一现象提醒小麦育种者应注意应用Pm21 的风险。来源于普通小麦与中间偃麦草（Thinopyrum intermedium）杂种后代的抗白粉病基因Pm40，被定位在小麦7BS 染色体上，对Bgt 具有广谱和持久的抗性。通过细胞学研究，并未在Pm40 的载体品种中发现大片段外缘染色体。过去几年的研究发现，Pm40 的载体品种具有优良的农艺性状。因此，我们相信在未来的育种工程中，Pm40 将会在Pm21 的抗性被克服之后起巨大的作用。另外，Pm21 和Pm40 都来源于外缘物种，这暗示着外源基因的抗性可能比小麦本身的基因更为持久和有效。

关键词： 小麦     白粉病     <i>Pm21i>     <i>Pm40i>     外源基因     内源抗性    

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NiFeRuO<i>xi> nanosheets on Ni foam as an electrocatalyst for efficient

《化学科学与工程前沿（英文）》 2023年 第17卷 第11期   页码 1698-1706 doi: 10.1007/s11705-023-2334-8

摘要： The electrocatalyst NiFeRuOx/NF, comprised of NiFeRuOx nanosheets grown on Ni foam, was synthesized using a hydrothermal process followed by thermal annealing. NiFeRuOx/NF displays high electrocatalytic activity and stability for overall alkaline seawater splitting: 98 mV@ 10 mA∙cm−2 in hydrogen evolution reaction, 318 mV@ 50 mA∙cm−2 in oxygen evolution reaction, and a cell voltage of 1.53 V@ 10 mA∙cm−2, as well as 20 h of durability. A solar-driven system containing such a bifunctional NiFeRuOx/NF has an almost 100% Faradaic efficiency. The NiFeRuOx coating around Ni foam is an anti-corrosion layer and also a critical factor for enhancement of bifunctional performances.

关键词： NiFeRuOx nanosheets     Ni foam     electrocatalysis     overall seawater splitting     solar-driven system    

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2014—2016年食品动物源RE-<i>cmeABCi>阳性弯曲菌流行与耐药性现状 Article

刘德俊, 刘蔚雯, 李星, 姚红, 沈张奇, 汪洋, 沈建忠

《工程（英文）》 2020年 第6卷 第1期   页码 34-39 doi: 10.1016/j.eng.2019.10.013

摘要：

弯曲菌是引起人类胃肠炎的一类重要食源性致病菌。RE-CmeABC是近年来弯曲菌中新发现的一种增强型耐药外排泵，可介导对兽医和人医临床治疗弯曲菌感染的重要药物，如氟喹诺酮类、酰胺醇类、大环内酯类及四环素类等药物的高水平耐药，而目前尚未有对该外排泵的大范围流行病学调查。因此，本研究调查了2014—2016年连续三年间我国山东、上海和广东等三个重点养殖区食品动物源RE-cmeABC阳性弯曲菌的流行与耐药性现状。结果显示，在1088株弯曲菌（931株结肠弯曲菌与157株空肠弯曲菌）中共检测出122株（11.2%）RE-cmeABC阳性菌株，包括111株（70.7%）空肠弯曲菌与11株（1.2%）结肠弯曲菌，空肠弯曲菌中RE-cmeABC的阳性率显著高于结肠弯曲菌。111株RE-cmeABC阳性空肠弯曲菌与46株RE-cmeABC阴性空肠弯曲菌相比在氟苯尼考、克林霉素和红霉素的耐药率上具有显著差异（P < 0.05），而在环丙沙星、四环素和庆大霉素的耐药率上无显著差异。然而，携带RE-cmeABC的菌株可以使环丙沙星与四环素对弯曲菌的MIC值分布趋于更高水平范围。脉冲场凝胶电泳分型（PFGE）结果显示RE-cmeABC阳性菌株在上海和广东地区以水平传播为主，在山东地区以克隆传播为主；三株分离于上海和广东的阳性菌为同一克隆型，4株分离于上海和山东的阳性菌为同一克隆型。本研究表明RE-cmeABC已在我国食品动物源弯曲菌中广泛流行，对公共卫生安全具有潜在威胁。

关键词： RE- <i> cmeABC i>     弯曲菌     环丙沙星     氟苯尼考    

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A highly efficient methodology for the preparation of

Yongxin Zhang, Shucheng Wang, Yaodong Huang

《化学科学与工程前沿（英文）》 2021年 第15卷 第3期   页码 679-686 doi: 10.1007/s11705-020-1979-9

摘要： A convenient and highly efficient method is described for the synthesis of -methoxycarbazole derivatives, including those with sterically demanding, benzannulated, or strongly electron-donating or -withdrawing substituents. Various -methoxycarbazole derivatives were directly prepared in good-to-moderate yields by the Pd (dba) CHCl /9,9-dimethyl-4,5-bis(diphenylphosphino)xanthene-catalyzed reactions of the corresponding dibromobiphenyl compounds and methoxya-mine. Based on this methodology, the first total synthesis of 3,3′-[oxybis(methylene)]bis(9-methoxy-9 -carbazole), an antimicrobial dimeric carbazole alkaloid previously isolated from the stem bark of , was achieved in 18% yield over seven steps from 1,2-dibromobenzene.

关键词： N-methoxyl carbazole     dimeric N-methoxyl carbazole     alkaloid     total synthesis     double N-arylation of methoxyamine    

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通过<i> B i>位Ta置换对<i> AB i> O4型EuNbO4的热学和力学性质进行优化 Article

陈琳, 冯晶

《工程（英文）》 2020年 第6卷 第2期   页码 177-184 doi: 10.1016/j.eng.2019.12.006

摘要：

铁弹性的ABO4型RETaO4陶瓷和RENbO4陶瓷（RE表示稀土）是具有潜在应用价值的热障涂层（TBC）材料，研究人员正在对其进行大量研究。结果显示， RETaO4陶瓷的力学性质优于RENbO4陶瓷的力学性质。在本研究中，我们通过B位钽（Ta）置换对利用固相法（SSR）制备的EuNbO4陶瓷的热学和力学性质进行优化。我们使用X射线衍射（XRD）法和拉曼光谱技术对所制备的晶体结构进行确认，并利用扫描电子显微镜（SEM）对结构表面的微观形貌进行观察。结果表明，通过B位Ta置换可以有效提高EuNbO4陶瓷的杨氏模量和热膨胀系数（TEC），且其最大值分别为169 GPa和1.12×10−5 K−1（1200 ℃）； EuNbO4陶瓷的热导率被降低至1.52 W·K–1·m–1（700 ℃），且材料的抗热辐射能力得到了改善。我们建立了声子热扩散系数与温度之间的关系，用以通过消除热辐射效应来确定声子本征热导率。研究结果表明，通过B位Ta置换能够有效优化EuNbO4的热学和力学性质，从而使得此种材料在将来可成为一种高温结构陶瓷材料。

关键词： 热障涂层，稀土铌酸盐，置换，热导率，热膨胀系数，杨氏模量    

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Traces and echoes of <i>De Architecturai> by Marcus Vitruvius Pollio in the work of Xu Guangqi in 17th

Michela CIGOLA,Yibing FANG

《机械工程前沿（英文）》 2016年 第11卷 第1期   页码 3-11 doi: 10.1007/s11465-015-0359-5

摘要：

This study aims to investigate the role played by Xu Guangqi (1562–1633), minister of the Ming Dynasty, in the development of European scientific and technical knowledge in China between the 16th and 17th centuries by analyzing a book of Western technology that he wrote, namely, Taixi Shuifa (On Western Hydraulics). Several Western books related to machine knowledge are searched to trace the source of the illustrations in Taixi Shuifa. We found that Archimedes’ screw and Ctesibius’ machine, which are included in Vitruvius’ De Architectura volumes, also appear in the work of Xu Guangqi.

关键词： Vitruvius’ De Architectura     Xu Guangqi’s Taixi Shuifa     Jesuit missionaries     Italian Renaissance     drawing     representation    

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Accounting for the uncertainties in the estimation of average shear wave velocity using – correlations

《结构与土木工程前沿（英文）》 2021年 第15卷 第5期   页码 1199-1208 doi: 10.1007/s11709-021-0749-1

摘要： Site-specific seismic hazard analysis is crucial for designing earthquake resistance structures, particularly in seismically active regions. Shear wave velocity ( V S) is a key parameter in such analysis, although the economy and other factors restrict its direct field measurement in many cases. Various V S–SPT– N correlations are routinely incorporated in seismic hazard analysis to estimate the value of V S. However, many uncertainties question the reliability of these estimated V S values. This paper comes up with a statistical approach to take care of such uncertainties involved in V S calculations. The measured SPT– N values from all the critical boreholes were converted into statistical parameters and passed through various correlations to estimate V S at different depths. The effect of different soil layers in the boreholes on the Vs estimation was also taken into account. Further, the average shear wave velocity of the top 30 m soil cover ( V S30) is estimated after accounting for various epistemic and aleatoric uncertainties. The scattering nature of the V S values estimated using different V S– N correlations was reduced significantly with the application of the methodology. Study results further clearly demonstrated the potential of the approach to eliminate various uncertainties involved in the estimation of V S30 using general and soil-specific correlations.

关键词： uncertainties     V S– N correlations     V S30     SPT data     statistical methodology    

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标题 作者 时间 类型 操作

Fluorescent properties and

Hong GAO,Jie SONG,Shibin SHANG,Zhanqian SONG

期刊论文

Recent advances in small molecule fluorescent probes for simultaneous imaging of two bioactive moleculesin live cells and <i>in vivoi>

Yongqing Zhou, Xin Wang, Wei Zhang, Bo Tang, Ping Li

期刊论文

Implications of static

John NSOR-ATINDANA, Maoshen CHEN, Liu WEI, Khin Myat NOE, Yue LI, Fang ZHONG

期刊论文

<i>Ni>-Positive ion activated rapid addition and mitochondrial targeting ratiometric fluorescent probesfor <i>in vivoi> cell H2S imaging

Yan Shi, Fangjun Huo, Yongkang Yue, Caixia Yin

期刊论文

SWIR Fluorescence Imaging <i>In Vivoi> Monitoring and Evaluating Implanted M2 Macrophages in Skeletal

Mo Chen, Yuzhou Chen, Sijia Feng, Shixian Dong, Luyi Sun, Huizhu Li, Fuchun Chen, Nguyen Thi Kim Thanh, Yunxia Li, Shiyi Chen, You Wang, Jun Chen

期刊论文

Effects of red and blue LEDs on

Lili CHEN, Xuzhang XUE, Yadong YANG, Fei CHEN, Jie ZHAO, Xiquan WANG, Alam Tariful KHAN, Yuegao HU

期刊论文

What will the future hold for artificial organs in the service of assisted reproduction: prospects and considerations

Mara Simopoulou, Konstantinos Sfakianoudis, Petroula Tsioulou, Anna Rapani, Polina Giannelou, Nikolaos Kiriakopoulos, Agni Pantou, Nikolaos Vlahos, George Anifandis, Stamatis Bolaris, Konstantinos Pantos, Michael Koutsilieris

期刊论文

Development of a new method for RMR and Q classification method to optimize support system in tunneling

Asghar RAHMATI,Lohrasb FARAMARZI,Manouchehr SANEI

期刊论文

抗白粉病基因 <i>Pm40i> 在我国“后 <i>Pm21i> 时代”小麦育种中的重要作用

唐圣雯,胡玉亭,钟胜福,罗培高

期刊论文

NiFeRuO<i>xi> nanosheets on Ni foam as an electrocatalyst for efficient

期刊论文

2014—2016年食品动物源RE-<i>cmeABCi>阳性弯曲菌流行与耐药性现状

刘德俊, 刘蔚雯, 李星, 姚红, 沈张奇, 汪洋, 沈建忠

期刊论文

A highly efficient methodology for the preparation of

Yongxin Zhang, Shucheng Wang, Yaodong Huang

期刊论文

通过<i> B i>位Ta置换对<i> AB i> O4型EuNbO4的热学和力学性质进行优化

陈琳, 冯晶

期刊论文

Traces and echoes of <i>De Architecturai> by Marcus Vitruvius Pollio in the work of Xu Guangqi in 17th

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期刊论文

Accounting for the uncertainties in the estimation of average shear wave velocity using – correlations

期刊论文